Deconstructive somatic cell nuclear transfer reveals novel regulatory T-cell subsets

I-12: Nuclear Reprogramming in Bovin Somatic Cell Nuclear Transfer

Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...

Efficiency of Ovine Fibroblast or Cumulus Cells For Somatic Cell Nuclear Transfer in Sheep

Purpose: Despite remarkable progresses have been achieved in the field of somatic cell nuclear transfer (SCNT), there is little information regarding the effect of donor cell type on the efficiency mammalian somatic cell cloning in vitro. This study compared in vitro developmental competency of sheep enucleated oocytes reconstructed with either fibroblast or cumulus cells. Material and methods...

Human somatic cell nuclear transfer (cloning).

Within 2 years of the announced birth in 1997 of Dolly, the lamb cloned from the mam-mary cells of an adult ewe, research groups announced that they had cloned mice and calves by using differentiated somatic cells (1– 3). In the cloning technique used to produce Dolly, the nucleus of a somatic cell of the ewe was transferred to a sheep oocyte from which the nucleus had been removed, and the cel...

The regulatory T cell family: distinct subsets and their interrelations.

Regulatory T Cell Subsets in Filarial Infection and Their Function

Filarial infections in humans are chronic infections that cause significant morbidity. The chronic nature of these infections with continuous antigen release is associated with a parasite-specific T cell hypo-responsiveness that may over time also affect the immune responses to bystander antigens. Previous studies have shown the filarial parasite antigen-specific T cells hypo-responsiveness is ...